Article
Modulation of translation initiation efficiency in classical swine fever virus.
Journal of virology - 1 Aug 2012
Friis Martin Barfred, Rasmussen Thomas Bruun, Belsham Graham J
Abstract excerpt
Modulation of translation initiation efficiency on classical swine fever virus (CSFV) RNA can be achieved by targeted mutations within the internal ribosome entry site (IRES). In this study, cDNAs corresponding to the wild-type (wt) or mutant forms of the IRES of CSFV strain Paderborn were amplified and inserted into dicistronic reporter plasmids encoding Fluc and Rluc under the control of a T7 promoter. The...
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