Article
Recombinant VirB5 protein as a potential serological marker for the diagnosis of bovine brucellosis.
Molecular and cellular probes - 1 Jun 2012
Tan Wei, Wang Xiu-ran, Nie Ying, Wang Chong, Cheng Li-qing, Wang Xiao-cen, Zhang Rui, Yan Guang-mou
Abstract excerpt
The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecular tag. Recombinant VirB5 was expressed and purified for evaluation as a diagnostic reagent for...
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