Article
Engineering rotor ring stoichiometries in the ATP synthase.
Proceedings of the National Academy of Sciences of the United States of America - 19 Jun 2012
Pogoryelov Denys, Klyszejko Adriana L, Krasnoselska Ganna O, Heller Eva-Maria, Leone Vanessa, Langer Julian D, Vonck Janet, Müller Daniel J, Faraldo-Gómez José D, Meier Thomas
Abstract excerpt
ATP synthase membrane rotors consist of a ring of c-subunits whose stoichiometry is constant for a given species but variable across different ones. We investigated the importance of c/c-subunit contacts by site-directed mutagenesis of a conserved stretch of glycines (GxGxGxGxG) in a bacterial c(11) ring. Structural and biochemical studies show a direct, specific influence on the c-subunit stoichiometry,...
Topics
- ATP Synthetase Complexes
- Adenosine Triphosphate
- Electrophoresis, Polyacrylamide Gel
- Microscopy, Atomic Force
- Microscopy, Electron
- Models, Molecular
- Mutation
- Protein Conformation
- Proteolipids