Article
Improved genetic transformation of cork oak (Quercus suber L.).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Alvarez-Fernández Rubén, Ordás Ricardo-Javier
Abstract excerpt
An Agrobacterium-mediated transformation system for selected mature Quercus suber L. trees has been established. Leaf-derived somatic embryos in an early stage of development are inoculated with an AGL1 strain harboring a kanamycin-selectable plasmid carrying the gene of interest. The transformed embryos are induced to germinate and the plantlets transferred to soil. This protocol, from adult cork oak to...
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