Article
Genetic analysis of the LexA repressor: isolation and characterization of LexA(Def) mutant proteins.
Molecular & general genetics : MGG - 1 Aug 1990
Oertel-Buchheit P, Lamerichs R M, Schnarr M, Granger-Schnarr M
Abstract excerpt
We report the isolation of LexA mutant proteins with impaired repressor function. These mutant proteins were obtained by transforming a LexA-deficient recA-lacZ indicator strain with a randomly mutagenized plasmid harbouring the lexA gene and subsequent selection on MacConkey-lactose indicator pl...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Codon
- Immunoblotting
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Repressor Proteins
- Serine Endopeptidases
- Transformation, Genetic
