Article
[Influence of temperature on spermatogenesis-related genes in spermatogonia cultured in vitro].
Zhonghua nan ke xue = National journal of andrology - 1 Feb 2012
Zhu Jing-Jing, Wang Da-Li, Li San-Hua, Deng Cheng-Liang, Sun Guang-Feng
Abstract excerpt
OBJECTIVE: To investigate the influence of temperature on the expressions of c-kit and PI3K in spermatogonia cultured in vitro at 32 degrees C and 37 degrees C, and provide basic scientific data for the mechanism of spermatogenic impairment due to body temperature (37 degrees C). METHODS: Isolated spermatogenic cells were cultured in vitro at 32 degrees C and 37 degrees C, and their adherence, proliferation and...
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