Article
Use of reverse transcription-real time polymerase chain reaction (real time RT-PCR) assays with Universal Probe Library (UPL) probes for the detection and genotyping of infectious pancreatic necrosis virus strains isolated in Chile.
Journal of virological methods - 1 Jul 2012
Calleja Felipe, Godoy Marcos G, Cárcamo Juan G, Bandín Isabel, Yáñez Alejandro J, Dopazo Carlos P, Kibenge Fred S, Avendaño-Herrera Ruben
Abstract excerpt
Reverse transcription-real time polymerase chain reaction (real time RT-PCR) assay with Universal Probe Library (UPL) probes has been developed for the detection and genotyping of Chilean infectious pancreatic necrosis virus (IPNV) isolates from infected cell culture. Partial nucleotide sequences (1175 bp) of the VP2 coding region from a selection of 7 Chilean IPNV isolates showed that they clustered into two...
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