Article
High resolution melt analysis to track infections due to ribotype 027 Clostridium difficile.
Journal of microbiological methods - 1 May 2012
Grando Danilla, Said Mohamed M, Mayall Barrie C, Gurtler Volker
Abstract excerpt
The increased prevalence of hypervirulent ribotype 027 Clostridium difficile requires rapid identification of isolates in order to implement timely infection control strategies. High resolution melt (HRM) analysis of PCR products can identify strain variation amongst genera of bacteria. The intergenic (16S-23S rDNA) spacer region contains sequence regions conserved within genera and other sequence region...
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