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Automating a combined composite-consensus method to generate DNA profiles from low and high template mixture samples.

Forensic science international. Genetics - 1 Sept 2012

Bekaert Bram, Van Geystelen Anneleen, Vanderheyden Nancy, Larmuseau Maarten H D, Decorte Ronny

Abstract excerpt

We present an automated method to generate DNA profiles from replicate PCRs by combining advantages of the composite and consensus method by a system of brackets in which an allelic balance threshold is used as a variable to separate DNA-profiles of major from minor donors. Through the analysis of artificial low (125 pg) and high (250 pg) template three-person mixtures with low (1:1.5:3) and high (1:5:10) donor...

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PMID: 22390852
DOI: 10.1016/j.fsigen.2012.02.001

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