Article
The ASB2β Ubiquitin-interacting motif is involved in its monoubiquitination.
Biochemical and biophysical research communications - 13 Apr 2012
Nishiyama Takehiro, Kuroda Sakiko, Takiguchi Eiko, Nakamura Takaaki, Hashimoto Keisuke, Tsuzuranuki Kenta, Kawakami Takao, Masuho Yasuhiko, Kohroki Junya
Abstract excerpt
ASB2 proteins are E3 ubiquitin (Ub) ligases that ubiquitinate filamins. There are two ASB2 splice variants, ASB2α and ASB2β. ASB2β has a ubiquitin-binding motif (UIM) at the N-terminal region but ASB2α does not. Here, we provide the first evidence that ASB2β but not ASB2α is monoubiquitinated and that this monoubiquitination involves the UIM. Myc-tagged ASB2β and hemagglutinin (HA)-tagged Ub were co-expressed in...
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