[Construction of the XZ113 delta eaeA, XZ113 delta stx2 and XZ113 delta ehxA mutants of STEC O18 XZ113 and their pathogenicity in mice].

Abstract excerpt

OBJECTIVE: To study the contribution of virulence genes of STEC O18 XZ113 isolate to the pathogenicity in mice. METHODS: The eaeA, stx2 and ehxA knock-out mutants of STEC strain XZ113 were generated using lambda-Red recombination system. RESULTS: Bacterial adherence test showed that the eaeA mutant adhered to HEp-2 cells in a diffuse manner with no microcolony formation. Vero cells assay showed that the stx...

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