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Article

Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Holme Inger Bæksted, Brinch-Pedersen Henrik, Lange Mette, Holm Preben Bach

Abstract excerpt

Agrobacterium-mediated transformation of in vitro cultured barley ovules is an attractive alternative to well-established barley transformation methods of immature embryos. The ovule culture system can be used for transformation with and without selection and has successfully been used to transform cultivars other than Golden Promise indicating minor genotype dependency. The method allows for the rapid and direct...

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PMID: 22351006
DOI: 10.1007/978-1-61779-558-9_13

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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