Article
Neurotoxin gene profiling of clostridium botulinum types C and D native to different countries within Europe.
Applied and environmental microbiology - 1 May 2012
Woudstra Cedric, Skarin Hanna, Anniballi Fabrizio, Fenicia Lucia, Bano Luca, Drigo Ilenia, Koene Miriam, Bäyon-Auboyer Marie-Hélène, Buffereau Jean-Philippe, De Medici Dario, Fach Patrick
Abstract excerpt
Clostridium botulinum types C and D, as well as their mosaic variants C-D and D-C, are associated with avian and mammalian botulism. This study reports on the development of low-density macroarrays based on the GeneDisc cycler platform (Pall-GeneDisc Technologies) applied to the simultaneous detection of the C. botulinum subtypes C, C-D, D, and D-C. The limit of detection of the PCR assays was 38 fg of total DNA,...
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