Cloning of a chromosomal fragment from Lactococcus lactis subsp. lactis partially complementing Escherichia coli recA functions.

doi:10.1016/0378-1097(90)90323-i

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Article

Cloning of a chromosomal fragment from Lactococcus lactis subsp. lactis partially complementing Escherichia coli recA functions.

FEMS microbiology letters - 1 Nov 1990

Novel M, Huang X F, Novel G

Abstract excerpt

A recA-like gene was isolated from a gene library of Lactococcus lactis subsp. lactis by intergeneric complementation of an E. coli recA mutant. A plasmid was obtained which fully complemented the RecA response to DNA damaging agents and UV inducibility of prophage, but not P1 plating efficiency in an E. coli recA mutant. The cloned DNA fragment also partially complemented the rec mutation in Lc. lactis MMS36....

Topics

Bacteriophage lambda
Chromosomes, Bacterial
Cloning, Molecular
DNA, Bacterial
Drug Resistance
Escherichia coli
Genes, Bacterial
Genetic Complementation Test
Genomic Library
Lactococcus lactis
Methyl Methanesulfonate

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mitomycin

Mitomycins

Mutation

Rec A Recombinases

Recombinant Proteins

Restriction Mapping

Ultraviolet Rays

Source

Open in PubMed

Identifiers

PMID: 2150659
DOI: 10.1016/0378-1097(90)90323-i

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2150659-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning of a chromosomal fragment from Lactococcus lactis subsp. lactis partially complementing Escherichia coli recA functions.PMID 2150659Read ↗

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