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Article

Rapid Identification of Monospecific Monoclonal Antibodies Using a Human Proteome Microarray

4 Feb 2012

Abstract excerpt

To broaden the range of tools available for proteomic research, we generated a library of 16,368 unique full-length human ORFs that are expressible as N-terminal GST-His(6) fusion proteins. Following expression in yeast, these proteins were then individually purified and used to construct a human proteome microarray. To demonstrate the usefulness of this reagent, we developed a streamlined strategy for the...

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PMID: 22307071
DOI: 10.1074/mcp.o111.016253

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