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Article

Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J.

Journal of bacteriology - 1 Nov 1990

Joerger M C, Klaenhammer T R

Abstract excerpt

Lactobacillus helveticus 481 produces a 37-kDa bacteriocin called helveticin J. Libraries of chromosomal DNA from L. helveticus were prepared in lambda gt11 and probed for phage-producing fusion proteins that could react with polyclonal helveticin J antibody. Two recombinant phage, HJ1 and HJ4, containing homologous inserts of 350 and 600 bp, respectively, produced proteins that reacted with antibody. These two...

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Identifiers

PMID: 2228964
DOI: 10.1128/jb.172.11.6339-6347.1990
PMCID: PMC526818

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