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Article

Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J.

Journal of bacteriology - 1 Nov 1990

Joerger M C, Klaenhammer T R

Abstract excerpt

Lactobacillus helveticus 481 produces a 37-kDa bacteriocin called helveticin J. Libraries of chromosomal DNA from L. helveticus were prepared in lambda gt11 and probed for phage-producing fusion proteins that could react with polyclonal helveticin J antibody. Two recombinant phage, HJ1 and HJ4, containing homologous inserts of 350 and 600 bp, respectively, produced proteins that reacted with antibody. These two...

Topics

Amino Acid Sequence
Bacteriocins
Base Sequence
Cloning, Molecular
Coliphages
DNA, Bacterial
Escherichia coli
Gene Expression
Genes, Bacterial
Genomic Library
Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Lactobacillus

Molecular Sequence Data

Open Reading Frames

Plasmids

Restriction Mapping

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Identifiers

PMID: 2228964
DOI: 10.1128/jb.172.11.6339-6347.1990
PMCID: PMC526818

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2228964-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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