ESR determinations of membrane permeability in a yeast sterol mutant.

doi:10.1016/0009-3084(79)90042-2

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Article

ESR determinations of membrane permeability in a yeast sterol mutant.

Chemistry and physics of lipids - 1 Jan 2000

Kleinhans F W, Lees N D, Bard M, Haak R A, Woods R A

Abstract excerpt

Yeast sterol mutants were subjected to ESR analysis in an attempt to elucidate how altered sterol composition correlates with membrane permeability. The technique requires spin labeling the intact yeast cells with a small, water-soluble nitroxide probe (2,2,5,5 tetramethyl-3-pyrrolin-1-oxyl-3-car...

Topics

Cell Membrane Permeability
Cell Survival
Electron Spin Resonance Spectroscopy
Gentian Violet
Mutation
Nickel
Saccharomyces cerevisiae
Spin Labels
Sterols

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