Article
ESR determinations of membrane permeability in a yeast sterol mutant.
Chemistry and physics of lipids - 1 Jan 2000
Kleinhans F W, Lees N D, Bard M, Haak R A, Woods R A
Abstract excerpt
Yeast sterol mutants were subjected to ESR analysis in an attempt to elucidate how altered sterol composition correlates with membrane permeability. The technique requires spin labeling the intact yeast cells with a small, water-soluble nitroxide probe (2,2,5,5 tetramethyl-3-pyrrolin-1-oxyl-3-carboxylic acid, PCA), suspending cells in a NiCl2 solution, and measuring the extent of Ni2+ entry through the membrane...
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