Article
Conditions affecting the activity of glucocerebrosidase purified from spleens of control subjects and patients with type 1 Gaucher disease.
Biochimica et biophysica acta - 18 Oct 1990
Aerts J M, Sa Miranda M C, Brouwer-Kelder E M, Van Weely S, Barranger J A, Tager J M
Abstract excerpt
Glucocerebrosidase was purified to homogeneity from spleens of control subjects and Type 1 Gaucher disease patients by immunoaffinity chromatography. Activation of the enzyme by taurocholate, phosphatidylserine and sphingolipid activator protein 2 (saposin C; SAP-2) was investigated by titration of combinations of various effectors in the absence and presence of Triton X-100. The specific activity of Type 1...
Topics
- Detergents
- Enzyme Stability
- Gaucher Disease
- Glucosylceramidase
- Humans
- Hydrogen-Ion Concentration
- Kinetics
- Mutation
- Octoxynol
- Phosphatidylserines
- Polyethylene Glycols