Article
Molecular characterization of three mutations in katG affecting the activity of hydroperoxidase I of Escherichia coli.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Loewen P C, Switala J, Smolenski M, Triggs-Raine B L
Abstract excerpt
Hydroperoxidase I (HPI) of Escherichia coli is a bifunctional enzyme exhibiting both catalase and peroxidase activities. Mutants lacking appreciable HPI have been generated using nitrosoguanidine and the gene encoding HPI, katG, has been cloned from three of these mutants using either classical probing methods or polymerase chain reaction amplification. The mutant genes were sequenced and the changes from...
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