Article
Molecular characterization of three mutations in katG affecting the activity of hydroperoxidase I of Escherichia coli.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Loewen P C, Switala J, Smolenski M, Triggs-Raine B L
Abstract excerpt
Hydroperoxidase I (HPI) of Escherichia coli is a bifunctional enzyme exhibiting both catalase and peroxidase activities. Mutants lacking appreciable HPI have been generated using nitrosoguanidine and the gene encoding HPI, katG, has been cloned from three of these mutants using either classical p...
Topics
- Base Sequence
- Catalase
- Chromosome Mapping
- Cloning, Molecular
- Enzyme Activation
- Escherichia coli
- Escherichia coli Proteins
- Hydrogen Peroxide
- Hydrogen-Ion Concentration
- Kinetics
- Molecular Sequence Data
- Mutation
- Nitrosoguanidines
- Spectrum Analysis
- Structure-Activity Relationship