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Article

A new efficient protocol for directed differentiation of retinal pigmented epithelial cells from normal and retinal disease induced pluripotent stem cells.

Stem cells and development - 10 Aug 2012

Zahabi Azadeh, Shahbazi Ebrahim, Ahmadieh Hamid, Hassani Seyedeh-Nafiseh, Totonchi Mehdi, Taei Adeleh, Masoudi Najmehsadat, Ebrahimi Marzieh, Aghdami Nasser, Seifinejad Ali, Mehrnejad Faramarz, Daftarian Narsis, Salekdeh Ghasem Hosseini, Baharvand Hossein

Abstract excerpt

We describe a new, efficient protocol that involves the serial addition of noggin, basic fibroblast growth factor (bFGF), retinoic acid, and sonic hedgehog (Shh) for the differentiation of human induced pluripotent stem cells (hiPSC) to retinal pigmented epithelium (RPE) in a serum- and feeder-free adherent condition. hiPSC-RPE cells exhibited RPE morphology and specific molecular markers. Additionally, several...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22145677
DOI: 10.1089/scd.2011.0599

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RPE

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