Article
Juvenile hormone action through a defined enhancer motif to modulate ecdysteroid-activation of natural core promoters.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology - 1 Mar 2012
Jones Grace, Jones Davy, Fang Fang, Xu Yong, New David, Wu Wen-Hui
Abstract excerpt
We have established a model system of hormone action, in an Sf9 cell transfection system, using defined enhancer motifs and natural core promoters of metamorphosis-associated genes. The DR1 enhancer, that is an established DNA binding site for the ecdysone receptor/ultraspiracle heterodimer, was necessary for transcriptional activation by 20-OH ecdysone. For this activated transcription, a natural sequence...
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