Article
In silico mining and characterization of 12 EST-SSRs for the invasive slipper limpet Crepidula fornicata.
Marine genomics - 1 Dec 2011
Riquet Florentine, Ballenghien Marion, Tanguy Arnaud, Viard Frédérique
Abstract excerpt
In silico mining of an expressed sequence tags (ESTs) library was found to be efficient at isolating simple sequence repeats (SSRs) loci in the non-indigenous marine mollusc Crepidula fornicata. Twelve SSR loci were developed for routine genotyping. Cross-species amplification to 8 other Crepidula species showed that the 12 loci are highly specific for C. fornicata. Mendelian inheritance was shown for 11 of them...
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