Article
Development of EST-SSRs by an efficient FIASCO-based strategy: a case study in rare minnow (Gobiocyrpis rarus).
Animal biotechnology - 1 Jan 2007
Cheng L, Liao X, Yu X, Tong J
Abstract excerpt
Type I markers are useful for comparative mapping and other genetic analyses, but relatively difficult to develop. In the present study a microsatellite (SSR)-enriched cDNA library was constructed for the first time using the fast isolation by AFLP of sequences containing repeats (FIASCO) method in a small fish, Chinese rare minnow (Gobiocypris rarus). A total of 97.4% of the expressed sequence tags (ESTs)...
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