Article
Development of EST-SSR markers by data mining in three species of shrimp: Litopenaeus vannamei, Litopenaeus stylirostris, and Trachypenaeus birdy.
Marine biotechnology (New York, N.Y.) - 1 Jan 2000
Pérez Franklin, Ortiz Juan, Zhinaula Mariuxi, Gonzabay Cesar, Calderón Jorge, Volckaert Filip A M J
Abstract excerpt
We report on the data mining of publicly available Litopenaeus vannamei expressed sequence tags (ESTs) to generate simple sequence repeat (SSRs) markers and on their transferability between related Penaeid shrimp species. Repeat motifs were found in 3.8% of the evaluated ESTs at a frequency of one repeat every 7.8 kb of sequence data. A total of 206 primer pairs were designed, and 112 loci were amplified with the...
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