The currently used commercial DNA-extraction methods give different results of clostridial and actinobacterial populations derived from human fecal samples

Abstract excerpt

Recently several human health-related microbiota studies have had partly contradictory results. As some differences may be explained by methodologies applied, we evaluated how different storage conditions and commonly used DNA-extraction kits affect bacterial composition, diversity, and numbers of human fecal microbiota. According to our results, the DNA-extraction did not affect the diversity, composition, or...

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