Article
Development of a biodegradable, temperature-sensitive dextran-based polymer as a cell-detaching substrate.
Macromolecular bioscience - 1 Jan 2012
Sun Guoming, Kusuma Sravanti, Gerecht Sharon
Abstract excerpt
A biodegradable, temperature-sensitive dextran-allyl isocyanate-ethylamine (TSDAIE) as a nonenzymatic cell detachment polymeric substrate for human endothelial progenitor cells (EPCs) is developed and examined. The lower critical solution temperature of TSDAIE is determined; its phase transition occurrs at 18 to 22 °C. For EPC culture, cell culture flasks are coated with TSDAIE and type I collagen. The TSDAIE...
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