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Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan-Based PCR Assay

1 Jul 2003

Abstract excerpt

We describe a rapid and reproducible PCR assay for quantitation of the Candida albicans ribosomal DNA (rDNA) in clinical blood samples based on the TaqMan principle (Applied Biosystems), in which a signal is generated by cleavage of a template-specific probe during amplification. We used two fluorogenic probes based on universal, fungus-specific primers, one for the detection of C. albicans species DNA and one...

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PMID: 12843077
DOI: 10.1128/jcm.41.7.3293-3298.2003

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