Rapid Identification of Commonly EncounteredCandidaSpecies Directly from Blood Culture Bottles

doi:10.1128/jcm.41.12.5660-5664.2003

Article

Rapid Identification of Commonly EncounteredCandidaSpecies Directly from Blood Culture Bottles

1 Dec 2003

Abstract excerpt

We report a rapid-cycle, real-time PCR method for identifying six Candida spp. directly from BACTEC blood culture bottles. Target sequences in the noncoding internal transcribed spacer regions of the rRNA operon were simultaneously amplified and interrogated with fluorescent probes to identify Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, and C. lusitaniae; these account for 88% of the...

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