Article
Minor groove recognition is important for the transcription factor PhoB: a surface plasmon resonance study.
Molecular bioSystems - 1 Nov 2011
Ritzefeld M, Wollschläger K, Niemann G, Anselmetti D, Sewald N
Abstract excerpt
The two-component regulatory system PhoR/PhoB induces the expression of several genes in response to phosphate starvation in Escherichia coli. In order to quantify these protein-DNA interactions and to study the time-resolved dynamics of the binding mechanism, the specific recognition of different oligonucleotide duplexes by the DNA-binding domain of PhoB (PhoB(DBD)) was analyzed using surface plasmon resonance....
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