Article
Measurement of in vivo expression of nrdA and nrdB genes of Escherichia coli by using lacZ gene fusions.
Molecular & general genetics : MGG - 1 Feb 1990
Gibert I, Calero S, Barbé J
Abstract excerpt
By using a promoter probe plasmid we investigated expression of the linked nrdA and nrdB genes coding for the two different subunits of the ribonucleoside diphosphate reductase enzyme of Escherichia coli. For this reason, nrdA-lacZ, nrdAB-lacZ and nrdB-lacZ fusions were constructed. Results obtained indicate that the nrdB gene has a promoter from which it may be transcribed independently of the nrdA gene....
Topics
- Base Sequence
- Cloning, Molecular
- DNA Damage
- DNA, Bacterial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Kinetics
- Lac Operon
- Molecular Sequence Data
- Mutation