Article
Isolation and characterization of mutants affected in the expression of the nar operon Escherichia coli.
Acta cientifica venezolana - 1 Jan 1990
Orozco de Silva A, Toro S
Abstract excerpt
In the nitrate reductase system of Escherichia coli, the maximal expression of the nar operon is obtained under anaerobiosis in the presence of nitrate. Mudl (Ap,lac) insertion mutants, which only grew on lactose anaerobically if supplemented with nitrate were mapped at the chlC locus at min 27 of the map. In these fusion strains which lack benzyl viologen dependent nitrate reductase (NR) activity as well as the...
Topics
- Chromosome Mapping
- Chromosomes, Bacterial
- Culture Media
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Lac Operon
- Mutation
- Nitrate Reductases
- Operon
