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The workflow for quantitative proteome analysis of chloroplast development and differentiation, chloroplast mutants, and protein interactions by spectral counting.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Friso Giulia, Olinares Paul Dominic B, van Wijk Klaas J

Abstract excerpt

This chapter outlines a quantitative proteomics workflow using a label-free spectral counting technique. The workflow has been tested on different aspects of chloroplast biology in maize and Arabidopsis, including chloroplast mutant analysis, cell-type specific chloroplast differentiation, and the proplastid-to-chloroplast transition. The workflow involves one-dimensional SDS-PAGE of the proteomes of leaves or...

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PMID: 21863448
DOI: 10.1007/978-1-61779-237-3_14

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