Article
Surveying the Oligomeric State of Arabidopsis thaliana Chloroplasts.
Molecular plant - 9 Jan 2017
Lundquist Peter K, Mantegazza Otho, Stefanski Anja, Stühler Kai, Weber Andreas P M
Abstract excerpt
Blue native-PAGE (BN-PAGE) resolves protein complexes in their native state. When combined with immunoblotting, it can be used to identify the presence of high molecular weight complexes at high resolution for any protein, given a suitable antibody. To identify proteins in high molecular weight complexes on a large scale and to bypass the requirement for specific antibodies, we applied a tandem mass spectrometry...
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