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Chloroplast phenomics: systematic phenotypic screening of chloroplast protein mutants in Arabidopsis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Lu Yan, Savage Linda J, Last Robert L

Abstract excerpt

As part of a project to analyze chloroplast functional networks systematically, we have subjected mutants in >3,200 nuclear genes predicted to encode chloroplast-targeted proteins in Arabidopsis thaliana (http://www.plastid.msu.edu) to parallel phenotypic assays. Detailed methods are presented for the various assays being used in this project to study chloroplast biology. These include morphological analysis of...

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PMID: 21863443
DOI: 10.1007/978-1-61779-237-3_9

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