Article
Localization of alpha 1----3-linked mannoses in the N-linked oligosaccharides of Saccharomyces cerevisiae mnn mutants.
Biochemistry - 13 Mar 1990
Alvarado E, Ballou L, Hernandez L M, Ballou C E
Abstract excerpt
Neutral and phosphorylated N-linked oligosaccharides were isolated from Saccharomyces cerevisiae mnn9 and mnn9 gls1 mutant mannoproteins and separated into homologues that differed in the number of terminal alpha 1----3-linked mannoses. In each type of oligosaccharide, the addition of such mannose was shown to occur in an ordered rather than a random fashion. The results confirm and extend an earlier report that...
Topics
- Carbohydrate Conformation
- Carbohydrate Sequence
- Chromatography, High Pressure Liquid
- Magnetic Resonance Spectroscopy
- Mannose
- Membrane Glycoproteins
- Mutation
- Oligosaccharides
- Phosphorylation
- Saccharomyces cerevisiae
