Article
Characterization of the defect in the Escherichia coli mutT1 mutator gene.
Journal of bacteriology - 1 May 1990
Bhatnagar S K, Bullions L C, Lew G, Bessman M J
Abstract excerpt
With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene.
Topics
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Restriction Mapping