Article
The gltF gene of Klebsiella pneumoniae: cloning and initial characterization.
Molecular & general genetics : MGG - 1 Oct 1991
Kuczius T, Eitinger T, D'Ari R, Castorph H, Kleiner D
Abstract excerpt
From a gene bank of Klebsiella pneumoniae M5a1, a 1.7 kb gene fragment was isolated which was able to restore the Ntr+ phenotype and ammonium (methylammonium) transport, but not glutamate synthase in an Escherichia coli glt mutant (glutamate synthase deficiency). The fragment strongly hybridized with the gltF regulatory gene from E. coli. After subcloning the fragment into an overexpression vector, a protein with...
Topics
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Genes, Bacterial
- Glutamate Synthase
- Klebsiella pneumoniae
- Mutagenesis, Insertional
- Mutation
- Restriction Mapping