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Article

A suitable duplex PCR for ovine embryo sex and genotype of PrnP gene determination for MOET-based selection programmes.

Reproduction in domestic animals = Zuchthygiene - 1 Dec 2011

Dervishi E, Sánchez P, Alabart J L, Cocero M J, Folch J, Calvo J H

Abstract excerpt

The objective of this study was to test the suitability of a duplex PCR assay for sex and scrapie resistance genotype determination in fresh embryos. Duplex PCR amplified a repetitive and specific fragment of Y chromosome, used for sex diagnosis, and a PrnP fragment. PrnP codons 134 and 156, and codon 171 were genotyped by restriction fragment length polymorphisms and allele-specific PCR, respectively, after...

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PMID: 21851426
DOI: 10.1111/j.1439-0531.2011.01774.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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