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Article

Determination of sex and scrapie resistance genotype in preimplantation ovine embryos.

Molecular reproduction and development - 1 Feb 2009

Guignot Florence, Baril Gerard, Dupont Francis, Cognie Yves, Folch Jose, Alabart Jose Luis, Poulin Naty, Beckers Jean-Francois, Bed'hom Bertrand, Babilliot Jean-Marc, Mermillod Pascal

Abstract excerpt

The aim of this study was to test the accuracy of genotype diagnosis after pre-amplification of DNA extracted from biopsies obtained by microblade cutting of ovine embryos and to evaluate the viability of biopsied embryos after vitrification/warming and transfer to recipients. Sex and PrP genotypes were determined. Sex diagnosis was done by PCR amplification of ZFX/ZFY and SRY sequences after PEP-PCR while PrP...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 18543282
DOI: 10.1002/mrd.20940

Genes

SRY
ZFX
ZFY

Provenance

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