Article
Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype.
Molecular & general genetics : MGG - 1 Nov 1991
Osterås M, Finan T M, Stanley J
Abstract excerpt
We have cloned and sequenced the pckA gene of Rhizobium sp. NGR234, a broad host-range strain. The gene encodes phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of gluconeogenesis. The locus was isolated and subcloned from a genomic library of NGR234 employing hybridization with an R. meli...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Fabaceae
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Gluconeogenesis
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Nucleic Acid Hybridization
- Phenotype
- Phosphoenolpyruvate Carboxykinase (GTP)
- Plants, Medicinal
- RNA, Bacterial
- RNA, Messenger