Article
A screening method for antifungal substances using Saccharomyces cerevisiae strains resistant to polyene macrolides.
The Journal of antibiotics - 1 Feb 1990
Etienne G, Armau E, Tiraby G
Abstract excerpt
Strains of Saccharomyces cerevisiae FL200 capable of growing on a solid medium containing a mixture of polyene macrolide antibiotics (nystatin, 40 micrograms/ml, amphotericin B, 40 micrograms/ml, pimaricin, 150 micrograms/ml and RP9971 antibiotic, 10 micrograms/ml) have been isolated after successive selection steps. The mutant strains, PR13 and PRC24, are 10 to 100 times more resistant than the polyene macrolide...
Topics
- Anti-Bacterial Agents
- Antifungal Agents
- Cycloheximide
- Drug Evaluation, Preclinical
- Drug Resistance, Microbial
- Ergosterol
- Macrolides
- Microbial Sensitivity Tests
- Mutation
- Polyenes
- Polysorbates