SYTO9 and SYBR GREEN1 with high resolution melting analysis for molecular confirmatory testing of the common Southeast Asian beta(0)-thalassemia mutations.

Abstract excerpt

Gel electrophoresis and ethidium bromide staining are routine methods in molecular laboratories. However, they are not ideally suited to large scale analyses in clinical laboratories. We used SYTO9 and high resolution melting (HRM) analyses for identification of the common beta(0)-thalassemia (beta(0)-thal) in Southeast Asia including the codons 17 (A>T), 41/42 (-TCTT) and 71/72 (+A) mutations. Multiplex...

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