Article
Development of 30 novel polymorphic Expressed Sequence Tags (EST)-derived microsatellite markers for the Miiuy Croaker, Miichthys miiuy.
International journal of molecular sciences - 1 Jan 2011
Xu Tianjun, Sun Dianqiao, Sun Yuena, Wang Rixin
Abstract excerpt
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 30 polymorphic microsatellite markers from 5053 ESTs of the Miichthys miiuy. Out of 123 EST derived microsatellites for which PCR primers were designed, 30 loci were polymorphic in 30 individuals from a single natural population with 2-13 alleles per locus. The observed and expected heterozygosities were from 0.1024 to...
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