Article
Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila.
Genetics and molecular research : GMR - 10 May 2017
Wang P P, Ren M, Chen S Q, Yin S W, Zhao C, Zhang H Y, Li X R, Cao Q Q, Zhou G Q
Abstract excerpt
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two individuals from a...
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