Article
Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila.
Genetics and molecular research : GMR - 10 May 2017
Wang P P, Ren M, Chen S Q, Yin S W, Zhao C, Zhang H Y, Li X R, Cao Q Q, Zhou G Q
Abstract excerpt
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two individuals from a...
Topics
- Alleles
- Animals
- Expressed Sequence Tags
- Genetic Markers
- Heterozygote
- Microsatellite Repeats
- Perciformes
- Polymorphism, Genetic
- Transcriptome
