Article
Gene expression and biochemical characterization of Azotobacter vinelandii cyclophilins and Protein Interaction Studies of the cytoplasmic isoform with dnaK and lpxH.
Journal of molecular microbiology and biotechnology - 1 Jan 2011
Dimou Maria, Venieraki Anastasia, Liakopoulos Georgios, Kouri Evangelia D, Tampakaki Anastasia, Katinakis Panagiotis
Abstract excerpt
The soil nitrogen-fixing bacterium Azotobacter vinelandii possesses two cyclophilins, comprising putative cytoplasmic and periplasmic isoforms, designated as AvPPIB and AvPPIA, respectively. Both recombinant cyclophilins have been purified and their peptidyl-prolyl cis/trans isomerase activity against Suc-Ala-Xaa-Pro-Phe-pNA synthetic peptides has been characterized. The substrate specificity of both cyclophilins...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Azotobacter vinelandii
- Cyclophilins
- Cytoplasm
- Gene Expression
- Isoenzymes
- Molecular Chaperones
- Mutation
- Oligopeptides
