Article
Arabidopsis cell-free extract, ACE, a new in vitro translation system derived from Arabidopsis callus cultures.
Plant & cell physiology - 1 Aug 2011
Murota Katsunori, Hagiwara-Komoda Yuka, Komoda Keisuke, Onouchi Hitoshi, Ishikawa Masayuki, Naito Satoshi
Abstract excerpt
The analysis of post-transcriptional regulatory mechanisms in plants has benefited greatly from the use of cell-free extract systems. Arabidopsis as a model system provides extensive genetic resources; however, to date a suitable cell-free translation system from Arabidopsis has not been available. In this study, we devised an Arabidopsis cell-free extract (ACE) to be used for in vitro translation studies....
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