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Discovery of polymorphisms in starch-related genes in rice germplasm by amplification of pooled DNA and deeply parallel sequencing.

Plant biotechnology journal - 1 Dec 2011

Kharabian-Masouleh Ardashir, Waters Daniel L E, Reinke Russell F, Henry Robert J

Abstract excerpt

High-throughput sequencing of pooled DNA was applied to polymorphism discovery in candidate genes involved in starch synthesis. This approach employed semi- to long-range PCR (LR-PCR) followed by next-generation sequencing technology. A total of 17 rice starch synthesis genes encoding seven classes of enzymes, including ADP-glucose pyrophosphorylase (AGPase), granule starch synthase (GBSS), soluble starch...

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1,4-alpha-Glucan Branching Enzyme
DNA, Plant
Glucose-1-Phosphate Adenylyltransferase
Glycoside Hydrolases
INDEL Mutation

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Starch Synthase

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PMID: 21645201
DOI: 10.1111/j.1467-7652.2011.00629.x

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