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Article

Basic techniques in human mesenchymal stem cell cultures: differentiation into osteogenic and adipogenic lineages, genetic perturbations, and phenotypic analyses.

Current protocols in stem cell biology - 1 Jun 2011

Bruedigam Claudia, Driel Marjolein van, Koedam Marijke, Peppel Jeroen van de, van der Eerden Bram C J, Eijken Marco, van Leeuwen Johannes P T M

Abstract excerpt

This unit describes basic techniques in human mesenchymal stem cell (hMSC) cultures. It includes protocols for the differentiation of hMSCs into osteogenic and adipogenic lineages, genetic perturbations, and phenotypic analyses. hMSCs can be differentiated with dexamethasone and β-glycerophosphate into mineralizing osteoblasts within 2 to 3 weeks, or with dexamethasone, indomethacin, and...

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PMID: 21633940
DOI: 10.1002/9780470151808.sc01h03s17

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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