Article
Molecular identification and analysis of nonserotypeable human enteroviruses.
Journal of clinical microbiology - 1 Apr 2010
Zhou Fei, Kong Fanrong, McPhie Kenneth, Ratnamohan Mala, Gilbert Gwendolyn L, Dwyer Dominic E
Abstract excerpt
Conventional approaches to characterizing human enteroviruses (HEVs) are based on viral isolation and neutralization. Molecular typing methods depend largely on reverse transcription-PCR (RT-PCR) and nucleotide sequencing of the entire or partial VP1 gene. A modified RT-PCR-based reverse line blot (RLB) hybridization assay was developed as a rapid and efficient way to characterize common and nonserotypeable (by...
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