Article
Application of high-resolution single-channel recording to functional studies of cystic fibrosis mutants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Cai Zhiwei, Sohma Yoshiro, Bompadre Silvia G, Sheppard David N, Hwang Tzyh-Chang
Abstract excerpt
The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic...
Topics
- Animals
- Cell Line
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- Electric Conductivity
- Humans
- Ion Channel Gating
- Kinetics
- Mice
- Models, Biological
- Mutation
- Patch-Clamp Techniques
- Probability